Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJM (1) Braz. J. Vet. Res. Anim. Sci. (1) Autor Andreolla,Ana Paula (1) Camargos,Marcelo Fernandes (1) Erpen,Luana Marina Scheer (1) Frandoloso,Rafael (1) Kreutz,Luiz Carlos (1) Leite,Rômulo Cerqueira (1) Lessa,Leandro Moreira (1) Reis,Jenner Karlisson Pimenta (1) Rocha,Maurílio Andrade (1) Stancek,Daniel (1) Mais... Palavra-chave Bovine leukemia virus (2) Diagnosis (1) ELISA (1) Gene (1) Immunodiffusion (1) Polymerase chain reaction (1) Retrovirus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development of a polymerase chain reaction and its comparison with agar gel immunodiffusion test in the detection of bovine leukemia virus infection Braz. J. Vet. Res. Anim. Sci. Camargos,Marcelo Fernandes; Stancek,Daniel; Lessa,Leandro Moreira; Reis,Jenner Karlisson Pimenta; Rocha,Maurílio Andrade; Leite,Rômulo Cerqueira. Polymerase chain reaction (PCR) was used for bovine leukemia virus (BLV) detection in the peripheral leukocytes of the infected bovines. The primers used were designed to amplify a part of env gene of BLV. PCR products were analyzed by agarose gel electrophoresis stained by ethidium bromide. The analytical specificity of PCR was confirmed by enzymatic restriction analysis of the PCR product with Bam HI and also by nucleotide sequence analysis of three PCR samples. Sixty five animals were tested for anti-BLV antibody, by agar gel-immunodiffusion test (AGID) and for direct BLV detection by PCR. There was a 73.80% concordance rate between the two tests. Four animals positive in AGID were PCR negative, while 13 AGID negative animals were found PCR positive.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunodiffusion; Bovine leukemia virus; Gene; Polymerase chain reaction. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500005 Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus BJM Andreolla,Ana Paula; Erpen,Luana Marina Scheer; Frandoloso,Rafael; Kreutz,Luiz Carlos. Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine leukemia virus; Retrovirus; Diagnosis; ELISA. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500068 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco